Low mannose-binding lectin and increased complement activation correlate to allograft vasculopathy, ischaemia, and rejection after human heart transplantation

Abstract

Aims: Transplant-associated coronary artery disease (TxCAD) is a major cause of post-transplant graft failure. The aim of this study was to investigate a possible role of mannose-binding lectin (MBL) deficiency and complement activation in TxCAD. Methods and results: In a prospective study of heart transplant recipients (n = 38) with a follow-up of 5.3 ± 1.3 years (range: 0.9-6.6), angiographically verified TxCAD (n = 6) was correlated to plasma MBL, complement activation, and endothelial activation (soluble E-selectin). MBL deficiency (<100 ng/mL) was detected in 3/6 patients with TxCAD and in 3/32 with non-TxCAD (Kaplan-Meier, P = 0.020). Furthermore, one or more acute rejection episodes were observed in 6/6 of the MBL-deficient patients and in 15/32 of the MBL-sufficient patients (χ2; P = 0.016). Complement activation (C4bc) correlated with soluble E-selectin (r = 0.36; P = 0.027), both being significantly higher in patients with ischaemia detected in the first biopsy (C4bc: 13.4 ± 6.1 AU/mL; E-selectin: 96 ± 13 ng/mL) than in those without ischaemia (C4bc: 6.3 ± 0.5; E-selectin: 51 ± 6; P = 0.037 and 0.002). Finally, terminal complement complex correlated closely with mortality (P = 0.002). Conclusion: Low MBL was related to the development of TxCAD and acute rejection and increased complement activation correlated to histopathologic ischaemia and mortality after heart transplantation. © The European Society of Cardiology 2005. All rights reserved.