Histogram specification as a method of density modification

Abstract

A new method for improving the quality and extending the resolution of Fourier maps is described. The method is based on a histogram analysis of the electron density. The distribution of electron density values in the map is forced to be `ideal'. The `ideal' distribution is assumed to be Gaussian. The application of the method to improve the electron density map for the protein Acinetobacter asparaginase, which is a tetrameric enzyme of molecular weight 140000 daltons, is described.