An efficient in vitro propagation of Aristolochia indica

Abstract

A rapid and efficient in vitro plant regeneration method was developed for Aristolochia indica. Multiple shoot formation was induced from shoot tip and nodal explants on Murashige and Skoog (MS) medium with 1-6 mg dm-3 2-isopentenyl-adenine (2-iP) or 1-4 mg dm-3 6-benzyladenine (BA). Maximum number of shoots were induced with 5 mg dm-3 2-iP alone (about 12-14 shoots). Shoot differentiation occurred directly from the leaf bases as well as from the internodes when cultured on 1-4 mg dm-3 BA and 0.8-2 mg dm-3 α-naphthaleneacetic acid (NAA) containing medium. Regeneration from the callus occurred when the calli initiated on MS medium containing 0.6-4 mg dm-3 NAA in combination with 0.8-3 mg dm-3 BA were transferred to 1-6 mg dm-3 BA alone containing medium. Elongated shoots were separated and rooted in MS medium containing 1 mg dm-3 indole-3-butyric acid. These were then transferred to soil after gradual acclimatization.