A comment on the analysis of bell-shaped dose-response curves

Abstract

A problem of the analysis of bell-shaped dose-response curves was theoretically discussed by using the response of ryanodine receptors for Ca2+ concentration as a model case. Usually the response curves were analyzed under the assumption that the receptor has a high affinity activation site and a low affinity inhibition site. However, a solution having a low affinity activation site and a high affinity inhibition site can be deduced theoretically from the same data. A method to avoid an erroneous result was proposed.