MicroRNA‑542‑3p represses OTUB1 expression to inhibit migration and invasion of esophageal cancer cells

Abstract

Dysregulation of microRBAs (miRBAs) is involved in the pathogenesis of esophageal cancer. miRBA (miR)-542-3p is a tumor suppressor in multiple types of cancer. However, whether and how miR-542-3p contributes to the progression of esophageal cancer remains unknown, and this is the aim of the present study. Un the current study, decreased expression of miR-542-3p was detected in tumor tissues compared with normal tissues from patients with esophageal cancer, and miR-542-3p expression was negatively correlated with mRBA expression levels of ovarian tumor domain-containing ubiquitin aldehyde-binding protein 1 (OTUB1) in tumor tissues from patients with esophageal cancer. Un KYSE150 human esophageal squamous cell carcinoma cells, overexpression of miR‑542‑3p significantly decreased OTUB1 at mRBA and protein levels, whereas downregulation of miR‑542‑3p significantly increased OTUB1 expression. Using a dual-luciferase assay, OTUB1 was validated to be a target gene of miR-542-3p in KYSE150 cells. Functionally, miR-542-3p significantly inhibited the migration and invasion of KYSE150 cells by repression of OTUB1 expression. These results demonstrated that miR-542-3p may promote the metastasis of esophageal cancer cells, and indicated that miR-542-3p may be a treatment target for esophageal cancer.