Aloe (Aloe barbadensis Miller) flour in broiler ration towards the ratio of Heterophyl Lymphocyte (H/L) and percentage of the relative weight of the lymphoid organ

Abstract

Phytobiotics had the immunomodulatory effects which known to increase the broiler immunity. Aloe contains acemannan and flavonoids with the effect of immunomodulator. The purpose of this study was to determine the optimal level of Aloe barbadensis Miller flour to improve broiler immunity. This study used a completely randomized design consisting of four treatments and four replications, T0: ration without aloe flour (control group), T1: ration + 0.075% aloe flour, T2: ration + 0.100% aloe flour, and T3: ration + aloe flour 0.125%. Eighty broilers were reared intensively and treatments given for 42 days. The ratio of heterophil/lymphocyte (H/L) and the percentage of the relative weight of lymphoid organ was determined at day-42. Results showed that aloe flour had a significant effect (P<0.01) on the ratio of heterophil/lymphocyte (H/L) and (P<0.05) on the percentage of the relative weight of bursa of Fabricius, but did not significantly affect the percentage of the relative weight of the spleen. The lowest (H/L) heterophil/lymphocyte ratio was T3 (0.29), and the highest was T0 (0.65). The highest relative weight percentage of the bursa of Fabricius was T3 (0.09%) and the lowest was T0 (0.05%). The relative weight percentage of the spleen was similar in group T0, T1 and T2, 0.12%, while T3 was 0.13% of the body weight. The addition of Aloe barbadensis Miller flour with a level of 0.125% (T3) in broiler ration tend to increase immunity through a decrease in the ratio of heterophil/lymphocytes (H/L) and increase in weight of bursa of Fabricius and spleen.