Prevalence and Molecular Characterization of Pseudomonas Species Isolated From Fish Markets in Port-Said

Abstract

Total number of 200 apparently healthy fish, Mugil cephalus and Oreochromis niloticus100 of each fish were collected randomly from different markets in Port-Said city to isolate and identify Pseudomonas spp. from it and the antibiotic sensitivity pattern of isolated strains. Pseudomonas was found in (66%) of Mugil cephalus and (80%) of Oreochromis niloticus. Four hundred and thirty six isolates identified as Pseudomonas spp. and classified into P. fluorescens (29.13%), P. aeruginosa (27.06%), P. putida (11.01%), P.cepacia (10.55%), P. stutzeri (8.49%), P. anguilliseptica (5.73%), P. alcaligenes (4.13%) and P. acidovorans (3.90%). The identification of Pseudomonas isolates were confirmed by Polymerase Chain Reaction which revealed that tested isolates were Pseudomonas and produced specific band at 618 bp, P. fluorescens produced specific band at 850 bp and P. aeruginosa produced electrophoresis at 956 bp. Antibiogram results showed that all Pseudomonas isolates were highly sensitive to Ciprofloxacin, Gentamicin and Chloramphenicol while were highly resistant to Ampicillin/sulbactam, Penicillin and Amoxicillin.