Enzymatic Synthesis of Oligosaccharides Containing Galβ→4Gal Disaccharide at the Non-Reducing End Using β-Galactanase from Penicillium citrinum

Abstract

The transglycosylation reaction was done with a β-galactanase from Penicillium citrinum. The regioselectivity in the transglycosylation reaction was studied using soy bean arabinogalactan as a donor and mono- or disaccharide derivatives containing β-galactosyl residue as acceptors. We also synthesized oligosaccharides containing Galβ1→4Gal sequence such as Galβ1→4Galβ1→4Glc, Galβ1→4Galβ1→3GlcNAc, Galβ1→4Galβ1→4GlcNAc, Galβ1→4Galβ1→6GlcNAc, and Galβ1→4Galβ1→3GalNAc for use in the total synthesis of complex sugar chains. © 1997, Taylor & Francis Group, LLC. All rights reserved.