Nonomuraea maheshkhaliensis sp. nov., a novel actinomycete isolated from mangrove rhizosphere mud

Abstract

A strain of Nonomuraea was isolated from Maheshkhall, Cox's Bazar, an unexplored region of Bangladesh. Strain 16-5-14T is a Gram-positive, aerobic, non-motile actinomycete that formed branched substrate and aerial mycelia. On the basis of 16S rRNA gene sequence similarity studies, strain 16-5-14T was shown to belong to the genus Nonomuraes, being most-closely related to Nonomuraea kuesteri. Chemotaxonomic date supported allocation of the strain as a member of the genus Nonomuraea. The strain 16-5-14T contained MK-9(H4) as the major menaquinone, the polar lipid was phosphatidylethanolamine and major cellular fatty acids were observed as C16:0 15.50%), iso-C16:0 (13.8%) and 10-methyl C17:0 (9.6%). Results of DNA-DNA hybridization and physiological tests allowed genotypic and phenotypic differentiation of strain 16-5-14T from closely related species N. kuesteri. Thus 16-5-14T represents a novel species of the genus Nonomuraea. On the basis of evaluation of the morphological, physiological and chemotaxonomic characteristics, 16S rRNA gene sequence comparisons and DNA-DNA hybridization, Nonomuraea maheshkhaliensis sp. nov. (type strain, 16-5-14T=JCM 13929T=MTCC 8545T) is proposed.