In vitro regeneration of Pinus brutia Ten. var. eldarica (Medw.) through organogenesis

Abstract

This paper describes two in vitro regeneration systems through direct and indirect organogenesis in Pinus brutia using fascicles aseptic cultures as explants. Mechanical scarification and gibberellic acid (GA3) were evaluated on in vitro seed germination. Scarification was the treatment that allowed for in vitro seed germination. The highest direct organogenic response was obtained in Murashige and Skoog (MS) medium containing 4.5 μΜ thidiazuron, whereas the highest indirect organogenesis was obtained with 9.8 μΜ thidiazuron and 3.4 μΜ paclobutrazol. The isolated shoots were rooted on MS medium supplemented with 1.70 μM indoleacetic acid. A large variation in root ability was observed among plantlets. These results suggest that both regeneration systems can be applied to the micropropagation or genetic transformation of P. brutia.   Key words: Pinus brutia, organogenesis, fascicles, paclobutrazol, thidiazuron