Antioxidant-polyphenols of saw palmetto seeds: statistical optimized production and improved functional properties under solid-state fermentation by Trichoderma reesei

Abstract

Saw palmetto seeds (SPS) contain essential phenolic compounds that provide antioxidant, antimicrobial, anti-inflammatory, and anti-diabetic benefits when added to food. Maximized/improved production of these valuable phenolic compounds is the main purpose of this study. Solid-state fermentation (SSF) is a promising processing technique that positively alters the levels of health-promoting compounds in plants and plant residues. Here, a central composite design matrix (16 runs) and response surface methodology were experimentally applied to investigate the best SSF conditions and their interactions for maximum production of phenolic compounds from SPS. A good correlation between actual and expected results was observed with higher multiple coefficients (R2 ~ 0.93–0.97) and strongly significant P values (< 0.0001) proving the accuracy of the statistical model/design. Under optimized SSF conditions, temperature 30 °C, moisture 10%, pH 7.0, and fermentation time 6 days, the total phenolic content and total antioxidant activity of SPS were maximized by 11-fold and 46–49 folds, respectively. According to HPLC analysis, the contents of all identifying polyphenols were 3.3–30.0 times greater in fermented SPS extract (FSPS) than in the unfermented SPS extract (UFSPS). The FSPS extract also contained four new/additional polyphenols (vanillic, p-coumaric, cinnamic, and quercetin). FSPS extract demonstrated much greater antibacterial and antifungal activities than UFSPS extract against various human pathogenic bacteria and fungi. Consequently, the FSPS-phenolic compounds can be exploited as a food supplement and an antimicrobial remedy.