Electroseparation of slaughterhouse by-product: Antimicrobial peptide enrichment by pH modification

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Abstract

The fractionation of bioactive peptides from hydrolysate is a main challenge to produce efficient alternative for synthetic additives. In this work, electrodialysis with ultrafiltration membrane (EDUF) was proposed to increase the purity of one antimicrobial peptide from slaughterhouse by-product hydrolysate. This targeted-peptide, α137–141 (653 Da, TSKYR), inhibits a large spectrum of microbial growths and delays meat rancidity; therefore, if concentrated, it could be used as food antimicrobial. In this context, three pH values were investigated during EDUF treatment to increase the α137–141 purity: 4.7, 6.5, and 9. pH 9 showed the highest purity increase—75-fold compared to the initial hydrolysate. Although the whole hydrolysate contains more than 100 peptides, only six peptides were recovered at a significant concentration. In this fraction, the α137–141 peptide represented more than 50% of the recovered total peptide concentration. The EDUF α137–141-enriched fraction obtained in this optimized condition would be a promising natural preservative to substitute synthetic additives used to protect food.

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Przybylski, R., Bazinet, L., Firdaous, L., Kouach, M., Goossens, J. F., Dhulster, P., & Nedjar-Arroume, N. (2020). Electroseparation of slaughterhouse by-product: Antimicrobial peptide enrichment by pH modification. Membranes, 10(5). https://doi.org/10.3390/membranes10050090

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