Fucoidan and Fucoidanase from Brown Seaweeds and Applications

Abstract

Fucoidan is a biologically active polysaccharide found in brown seaweeds. The present study was carried out to isolate and investigate the biological activities of fucoidan and the activity of fucoidanase (EC: 3.2.1.44) from Padina pavonica, Sargassum latifolium and Colpomenia sinuosa. C. sinuosa expressed the highest fucoidan content and the highest fucoidanase activity. The isolated fucoidan from C. sinuosa expressed appreciable anticoagulant and antibacterial activities against Bacillus sp, Pseudomonas aeruginosa, Echerichia coli, Staphyllococcus aureus and Proteus vulgaris. It also showed antioxidant activity using DPPH [DPPH (1,1-diphenyl-2-picrylhydrazyl)] and ABTS [2, 2-azinobis (3- ethylbenzothiazoline- 6-sulfonic acid)] methods. Fucoidan from C. sinuos inhibited both α- glucosidase (EC: 3.2.1.20) and α-amylase (EC: 3.2.1.1). The inhibition of fucoidan for α- glucosidase activity was stronger than that of α-amylase activity. The results suggest that fucoidan from C. sinuosa can be used as antibacterial, antioxidant and antidiabetic