Opto-structural Characteristics and Biomedical Applications of Microwave Irradiated Green Synthesized AM-AgNP from Atalantia monophylla (L.) Leaf Extract

Abstract

The present study focused on the biocompatible, nano-sized, Atalantia monophylla aqueous leaf extract synthesized silver nanoparticles (AM-AgNP), with significant biomedical applications. Surface Plasmon Resonance (SPR) of AM-AgNP recorded at 396 nm wavelength at an optimum 12 pH and the impeccable intensity of emission peak obtained at 513 nm of Photoluminescence (PL) spectra at 400 nm excitation wavelength, endorsed better synthesis and maximum quantum yield respectively. AM-AgNP atom arrangement exhibited face-centered cubic (FCC) unit type, with an average crystal size of 8.3 nm, and when combined formed a polycrystalline particle size of 11.3 nm, as calculated by Scherer’s formula using X-ray Diffraction (XRD) graph, High-Resolution Transmission Electron Microscope (HR-TEM) micrograph and Selective Area Electron Diffraction (SAED) pattern respectively. Biocompatible silver nanoparticles have shown selective antimicrobial activity against gram-positive and gram-negative bacterial strains. Green synthesized AM-AgNPs have shown significant in-vitro removal of 2, 2-diphenyle-1-picrylhydrazyl (DPPH) and 2,2-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) free radicals compared to aqueous A. monophylla leaf extract and Gallic acid standard. Cell cytotoxicity of AM-AgNP was evaluated against normal (immortal) human embryonic kidney (HEK 293) cell lines and human cervical cancer (HeLa) cell lines that revealed dose-dependent, selective cell cytotoxicity besides biocompatible properties.