EVLncRNAs 3.0:ãn updated comprehensive database for manually curãt ed functional long non-coding RNAs validat ed b y lo w-throughput experiments

Abstract

Long noncoding RNAs (lncRNAs) ha v e emergedãs crucial regulatorsãcross diverse biological processesãnd diseases. While high-throughput sequencing has enabled lncRNA disco v ery, functional characterization remains limited. The EVLncRNAs database is the firstãnd exclusive repos- itory forãll experimentally validated functional lncRNAs from various species. After previous releases in 2018ãnd 2021, this update marksã major expansion through exhaustive manual curation of nearly 25 0 0 0 publications from 15 May 2020, to 15 May 2023. It incorporates substan- tial growthãcrossãll categories:ã 154% increase in functional lncRNAs, 160% inãssociated diseases, 186% in lncRNA-diseaseãssociations, 235% in interactions, 138% in str uct ures, 234% in circular RNAs, 235% in resistant lncRNAsãnd 4724% in e x osomal lncRNAs. More im- portantly, it incorporatedãdditional information include functional classifications, detailed interaction pathwã y s, homologous lncRNAs, lncRNA locations, COVID-19, phase-separationãnd organoid-related lncRNAs. The web interface was substantially improved for browsing, visualization,ãnd searching. ChatGPT was tested for information extractionãnd functional overview with its limitation noted. EVLncRNAs 3.0 represents the most e xtensiv e curated resource of experimentally validated functional lncRNAsãnd will serveãsãn indispensable platform for unravelling emerging lncRNA functions. The updated database is freelyãvailableãt https://www.sdklab- biophysics- dzu.net/ EVLncRNAs3/ .