Genetically engineered fertile indica-rice recovered from protoplasts

Abstract

We have established an efficient protocol for plant regeneration from haploid In-dica-type rice protoplasts. Incubation of these protoplasts with the selectable hy-gromycin phosphotransferase (hph) gene expressed under control of the 35S promoter of cauliflower mosaic virus (CaMV) and polyethyleneglycol (PEG), and subsequent culture in the presence of hygromy-cin B, led to the recovery of numerous resistant clones from which 77 plants were regenerated. Data from Southern analysis and enzyme assays proved that the trans-gene was stably integrated into the host genome and expressed, and that it was inherited in offspring. © 1990 Nature Publishing Group.