Relationship between superoxide dismutase manganese gene polymorphism and eye tumors

Abstract

BACKGROUND: Orbital tumor in Indonesia is one of the eye health problems that can cause blindness. The impact caused by orbital tumors on patients is quite large because it can result in blindness and even death due to its metastatic nature. The role that superoxide dismutase (SOD) plays in cancer biology is not well understood, most studies showing a more oxidative state, characterized by increased intracellular reactive oxygen species, particularly superoxide. AIM: The objective of the study was to determine the relationship between manganese SOD2 gene polymorphisms and the incidence of orbital tumors in Medan. METHODS: This study is an analytic observational study with a cross-sectional data collection method using controls. Comparisons were made between the control group and the observed group to see the relationship between SOD2 polymorphisms and the risk of orbital tumor incidence in Medan. The ophthalmic examination, anterior and posterior segments, and assessment of computed tomography orbit if deemed necessary for the orbital tumor patients. Histopathological examination was done by the Pathologist. Blood samples were taken for polymorphism examination on extracted DNA using the Polymerase Chain Reaction and Restriction Fragment Length Polymorphism methods. RESULTS: About 30 patients that met the inclusion criteria. Laterality, the left eye is more likely to suffer from tumors when compared to the right eye. This study found as many as 16 patients, while malignant tumors were 14 patients. There was a relationship between the MnSOD gene polymorphism and the incidence of orbital tumors (P < 0.001), there was a relationship between the MnSOD gene polymorphism and the incidence of orbital tumors in the female sex (P < 0.001). CONCLUSION: There was a relationship between MnSOD gene polymorphisms and the incidence of orbital tumors (P < 0.001).