Direct protein identification from nonspecific peptide pools by high-accuracy MS data filtering

Abstract

Validate your results! High-accuracy mass spectrometric analysis and composition-based de novo sequencing (CBS) combined with database(DB)-assisted protein identification allows for the rapid production of validated analytical results in proteome research. A simplified sample preparation scheme is sufficient to provide for reliable identification of proteins even when following nonspecific and unknown degradation processes. (Figure Presented). © 2006 Wiley-VCH Verlag GmbH & Co. KGaA.