EFFECTIVENESS OF CHITOSAN-BASED 0.5% TETRACYCLINE GEL AND FREEZE-DRIED PLATELET-RICH PLASMA ON POST-PERIODONTAL SURGERY HEALING: CLINICAL AND IMMUNOHISTOCHEMICAL EVALUATION

Abstract

Objective: Periodontal treatment widely uses tetracycline as a local antibiotic because it has broad-spectrum antibacterial and anticollagenase properties. Chitosan-based 0.5% tetracycline gel, there was an increase in the performance of tetracycline because chitosan released the drug locally, was slower, controlled, lasted longer, had better stability and lower toxicity. Periodontal treatment is currently trending using Platelet-Rich Plasma (PRP) because of its properties, which release many growth factors. To facilitate clinical administration, PRP was developed by the freeze-drying process to become allogeneic Freeze Dried PRP (FD-PRP) obtained from the blood bank. Combination can help accelerate the healing of periodontal surgical wounds. To evaluate the effectiveness of administration of chitosan-based 0.5% tetracycline gel and FD-PRP. Methods: Laboratory experimental study with posttest-only control group design on 30 Wistars-induced periodontitis. The sample was divided into 5 groups: negative and positive control group and 3 periodontal surgical intervention groups accompanied by drug administration. The examination of clinical parameters such as Bleeding on Probing (BoP) and Pocket Depth (PD) as well as immunohistochemical parameters such as fibroblast growth factor-2 (FGF-2) were evaluated on days 3 and 14. Results: This study showed a significant decrease in BoP on day 3 (p=0.022) and 14 (p=0.009), decrease in PD on day 14 (p=0.007) and a significant increase in FGF-2 on day 3 (p=0.024) and decrease on day 14 (p=0.017). Conclusion: Administration of chitosan-based 0.5% tetracycline gel and FD-PRP were effective in periodontal surgical wound healing because it reduced BoP and PD and increased fibroblast cell proliferation through increasing FGF-2 expression.