Dynamic of bacterial diversity in ileum digesta under water supplements of antibiotics and probiotics

Abstract

Background and Objective: Intestinal bacteria plays an important role in the physiological and immunological status of birds. The present study investigated the diversity of intestinal bacterial of broilers that were fed corn-soy diet and supplements of antibiotics and probiotics. Materials and Methods: The supplemented treatments were water, water with antibiotics, water with probiotic and water with antibiotics and probiotic. Eight randomly sampled broilers were slaughtered and their ileum digesta taken. The total of 32 samples was then subjected for DNA extraction. Subsequently, bacterial tag-encoded 16 FLX ampliconpyro sequencing (bTEFAP) procedures were performed. Results: Nearly all treatments samples contained Lactobacillus, with the majority related to water with probiotic treatment. In contrast, different pathogenic bacteria along with Lactobacillus observed for other three treatments. Conclusion: The probiotics supplements made a shift towards useful bacterial species; Lactobacillussalivarius and Lactobacillusaviarius. This study promoted for replacing the probiotics as a prophylaxis in gastrointestinal infection and diseases instead of antibiotics.