Role of xylem in root hydraulics: Functionality and implications for drought adaptation

Abstract

Root water transport has been viewed as primarily limited by the radial component, with the axial pathway considered highly conductive and non-limiting. This is supported by theoretical estimates of axial conductance using the Hagen–Poiseuille equation. However, increasing evidence indicates that actual axial conductance is often nearly an order of magnitude lower than predicted, challenging assumptions that it does not limit water uptake. In this review, we discuss how recent model inversion approaches, guided by root hydraulic conductance measurements, have revealed that water transport can be co-limited by radial and axial conductance. We explore possible explanations for this co-limitation, with particular attention to root topology. Finally, we highlight how drought-induced adjustments in xylem vessel traits can reduce axial conductance, contributing to water conservation and cavitation resistance, thereby supporting drought adaptation strategies. Leveraging this overlooked limitation opens new avenues for breeding crops with improved water-use efficiency and resilience to drought.