Isolation and Characterization of Monoclonal Antibody Directed Against Bovine αs2-Casein

Abstract

A monoclonal antibody 62-1A of isotype IgM, directed against bovine αs2-casein, was isolated and characterized. Monoclonal antibody 62-1A recognized bovine αs2-11P- and αs2-9P-caseins by indirect solid phase radioimmunoassay and Western blot analysis. Crossreactivity toward native genetic variants (A, B, and C) of αs1-casein was similar but lower (˜60 to 70%) than that for αs2-11P-casein). Little or no crossreactivity was observed for other bovine milk or serum proteins. Antibody affinity for αs2-11P-casein was 1.3 × 109/M. As little as 25 ng/ml (.5 ng/well) of αs2-11P-casein was detected by solid phase radioimmunoassay. © 1991, American Dairy Science Association. All rights reserved.