Implementing stepwise solvent elution in multisyringe liquid chromatography (MSC) to separate norepinephrine, epinephrine and dopamine

Abstract

A multisyringe liquid chromatographic (MSC) method by using stepwise solvent elution and reverse-phase liquid chromatography C 18 monolithic column to separate Epinephrine (E), Norepinephrine (NE) and Dopamine (DA) was developed. The first step employs the mixture sodium1-hexanosulfonate monohydrate (HS) 7.5mM: methanol (98:2) to separate NE and E and the second step uses HS (5.0 mM): methanol (92:8) to elute DA. The three analytes were separated on 6 min spending only 10 mL of mobile phases. The detection limits were between 1.3 μg mL -1 (DA) and 2.04 μg mL -1 (NE). © 2012, Sociedad Química de México.