Poly aromatic hydrocarbons degradation by lignin peroxidase produced from local isolate Aspergillus terreus SG777

Abstract

Lignin peroxidase produced from A. terreus SG777 precipitated with 80% ammonium sulfatesaturation then desalting by dialysis against Tris buffer solution and partially purified by ionexchange chromatography using DEAE- cellulose.The specific activity reached 56.67U/mg withpurification fold was 5.4 times and 13.7% recovery. The optimum pH for activity and stability was(3). The maximum activity was observed at 40°C and the enzyme maintained the activity when itwas incubated at (25-35)˚C.Degradation of poly aromatic hydrocarbons when incubated with 65.51 U/ml lignin peroxidaseat (40°C) for 4 hr compared with the Refinery treatment. Results showed (97.54%) removalefficiency of Di benzo (a, h) anthrancene by lignin peroxidase, while only (54.37%) was treated byRefinery treatment. Flourene and anthrancene degradation (95.98%) and (83.51%) respectively,while the flourene and anthrancene degradation treated by Refinery treatment only 84.51% and(46.83%) respectively.The aim of this study was degradation of poly aromatic hydrocarbons by using partialpurification lignin peroxidase.