Extended Spectrum Beta- Lactamase- Producing Uropathogenic Escherichia coli in Pregnant Women Diagnosed With Urinary Tract Infections in South-Western Nigeria

Abstract

Extended spectrum beta-lactamase (ESBL)-producing uropathogenic Escherichia coli (UPEC) in symptomatic pregnant women with confirmed urinary tract infections in Southwest. Nigeria was reported. Susceptibility of UPEC isolates to β-lactam and other classes of antibiotics was determined by the Kirby-Bauer’s disc diffusion method on Mueller-Hinton agar plate. Detection of plasmid DNA in multiple antibiotic resistant isolates was carried out by alkali lysis (TENS) method. Extended-spectrum-β-lactamase (ESBL) production was determined by double disk synergy test (DDST). Isolates that were positive to ESBL were mated with non-ESBL- producing E. coli and other enterics in a conjugation experiment. Transfer of ESBL-enzyme and other resistance phenotypes in the transconjugants was investigated by DDST. Data obtained were statistically analyzed using SPSS 17. Greater percentage of the isolates were multiple antibiotic resistant (MAR). Sixty-nine (26.1 %) of UPEC were ESBL producers. Some of the ESBL producers transferred ESBL- enzyme and other resistance determinants to the recipients. Large size plasmid DNA of molecular weight (23.13-33.04 kb) was detected in some representative MAR isolates.