Detection of Flexibacler psychrophiius by immunofluorescence in fish, suffering from fry mortality syndrome: a rapid diagnostic method

Abstract

A rapid diagnostic method for fry mortality syn-drome is described. The method is based on immunofluores-cence analysis of spleen imprints from diseased rainbow trout fry and utilizes rabbit antlserum prepared against Flexibacter psychrophilus. A low level of cross reactivity with Flexibacter columnans was observed but this can be avoided by absorb-ing the diagnostic antiserum with F columnans.