Studies on lipoprotein X: Comparison of the methods of detection and its clinical applications

Abstract

Comparative studies on the method of lipoprotein X (LP-X) detection were performed for the purpose of its clinical application. The method based on the peculiar property of LP-X which migrated toward the cathode by electrophoresis on agar-gel. LP-X was visualized after the electrophoresis by each one of the following methods; (1) Precipitation with specific antiserum which was prepared in our laboratory, (2) Precipitation with dextran sulfate-Ca++, (3) Staining with sudan black B. The sensitivity of the LP-X detection depended on the selection of agar and the preparation of agar-gel but not on the methods of visualization. LP-X was detected in various kinds of disease with intrahepatic or extrahepatic cholestasis. In 85 LP-X positive patients, 5 cases (6%) was found with normal level of serum bilirubin, alkaline phosphatase total cholesterol and GOT. © 1975, The Japan Society of Hepatology. All rights reserved.