Structural determination and DPPH radical-scavenging activity of two acylated flavonoid tetraglycosides in oolong tea (Camellia sinensis)

Abstract

Two major acylated flavonoid tetraglycosides were isolated from the methanol extract of oolong tea. Their structures were elucidated by spectroscopic methods as quercetin 3-O-[2G-(E)-coumaroyl-3 G-O-β-D-glucosyl-3R-O-β-D-glucosylrutinoside] (1) and kaempferol 3-O-[2G-(E)-coumaroyl-3G-O-β-D- glucosyl-3R-O-β-D-glucosylrutinoside] (2). Compounds 1 and 2 exhibited scavenging activity against DPPH radical with EC50 values of 30.5 and 487.2 μM, respectively. © 2008 Pharmaceutical Society of Japan.