Probiotic research in several products of virgin coconut oil from Padang, Indonesia

Abstract

Virgin Coconut Oil (VCO), have been produce by comunity people in Padang West Sumatra, Indonesia, with diffrent quality and the process. Most of the process using spontaneos fermentation methods with diffrent location of making and environment. We are interested in probiotic research in VCO samples to find good or new Lactic Acid Bacteria (LAB) as probiotic and antimicrobial to maintain good health. There were 7 VCO sampels have been taken from local market that have been isolated and characterized antimicrobial activity and molecular spesies. The were 11 LAB colonies have been found. The majority of LAB samples were good inhibition zone agains indicator bacterias such as, Escherichia coli, Staphylococcus aureus and Bacillus subtilis. Ampicillin Sodium 100 μg/mL was used as positive control. The results shows the inhibition zone was obtained from sample 1A (12 mm), agains E.coli, sample 6C (13.5 mm) for Bacillus and 3A (15 mm), for S. aureus as at low pH from 3 to 5and high temperature from 60 to 100 degee Celcius. Ssequence analysis of 11 isolates are diversity of Lactobacillus plantarum species with different strains and new diversity of Lactobacillus sakei.