Optimization and Physico-Chemical Characterization of a Bacteriocin Produced By Marine Lactobacillus Rhamnosus L43

Abstract

Objective: To optimize bacteriocin producing medium and their characterization. Methods: Lactobacillus strain was isolated from marine water, Thirumullavaram beach, Kerala using MRS broth (Hi-Media, India) at 37 0 C for 48 hrs. The isolate was identified by 16S rRNA sequencing and phylogenetic analysis. Optimization of physiological parameters and carbon, nitrogen sources. Produced crude bacteriocin and antagonistic characteristics of bacteriocin against Klebsiella pneumoniae MTCC535 were studied by agar well diffusion method. Bacteriocin stability in different temperature and enzyme. Result: Lactobacillus rhamnosus strain isolated from marine water. Lactobacillus isolate was sequenced which shown high similarity with reference strain Lactobacillus rhamnosus ZY Accession number KC012630.1. Bacteriocin produced at 30 0 C, pH 7, 18 hrs. incubation time with 2% inoculum size and 2% NaCl concentration. It produced in presence of lactose and peptone. All the data validated by statistical analysis using paired t-test by MINITAB 14. This bacteriocin was stable at 100 0 C for 20 min, and it is in proteinaceous nature. Conclusion: These results indicated the potent strain produce bacteriocin. Maximum bacteriocin production was observed at 30 o C, 18 hrs. incubation, pH 7.0, 2% NaCl solution.