Characterization of antioxidative fraction of plant stem Bouea macrophylla Griff

Abstract

Ethyl acetate extract of gandaria (Bouea macrophylla Griff) has very strong antioxidant activity. The aims of this study are to separate and characterize the active fraction of antioxidants and determine the antioxidant activity quantitatively. Ethyl acetate extract of B. macrophylla was separated by gravity column chromatography. Fraction separating was guided with qualitative testing of antioxidant activity. Active fraction result of separation was characterized by liquid chromatography-mass spectroscopy and quantitatively analyzed antioxidant activity using the 2.2-diphenyl-1-picrylhydrazyl method. Naringenin and luteolin were identified in the D-2 fraction which had very strong antioxidant activity with an IC50 value of 2.13 ppm. The hydroxyl group OH group of naringenin and luteolin compounds was thought to play a role in the reduction of free radicals.