Polyherbal EMSA ERITIN blocks nuclear factor-Kappa B (NF-κB) and proinflammatory cytokines in irradiated mice

Abstract

Radiationtherapy is commonly used in cancer treatment that produce deleterious effects,such as normal cell death (apoptotic or necrotic) and suppression ofhematopoietic stem cells. This effect was caused by ion rays that can rise tohigh of free radical. The high of free radical trigger the inflammation ofcells. EMSA ERITIN is medicinal herb used as preventative agents or radioprotectiveto reduce the deleterious effects of radiation. EMSA ERITIN contains soy, redrice and coconut water extract. This study aimed to analyze the effect of polyherbalEMSA ERITIN to the expression of transcription factor of inflammation (NF-κB).We also observe the proinflammatory cytokines (TNF-α and IFN-γ) in BALB/c miceafter radiation. Polyherbal EMSA ERITIN grouped into three doses: Low dose(1.04 mg g -1 BW), optimum dose (3.125 mg g -1 BW) and high dose (9.375 mg g -1 BW). The radiation dose that applied to mice is 5 Gy. The oraladministration of EMSA ERITIN started 24 h after radiation and continued until2 weeks. Data were analyzed using One-way ANOVA (p<0.05) and Tukey testusing SPSS 16.0 for Windows. The result showed that the level of NF-κB, TNF-αand IFN-γ in irradiated mice significantly decreased when the mice receivedEMSA ERITIN. All dose of polyherbal EMSA ERITIN showed more effective than EPO(Hemapo Epoetin alfa ™) treatment to reduced TNF-α, IFN-γ and NF-κB. So that,EMSA ERITIN is one of potential herbal medicine act as a preventive agent orradioprotective after radiation.