Slug Is a Novel Downstream Target of MyoD

Abstract

Temporal expression profiling was utilized to define transcriptional regulatory pathways in a mouse muscle regeneration model. Potential downstream targets of MyoD were identified by temporal expression, promoter data base mining, and gel shift assays; and were identified as novel MyoD targets. , a member of the snail/slug family of zinc finger transcriptional repressors critical for mesoderm/ectoderm development, was further shown to be a downstream target by using promoter/reporter constructs and demonstration of defective muscle regeneration in null mice.