DNA-induced synthesis of biomimetic enzyme for sensitive detection of superoxide anions released from live cell

Abstract

In this work, we successfully fabricate a rapid, sensitive sensor for the detection of superoxide anions O2- based on graphene/DNA/Mn3(PO4)2 biomimetic enzyme. In the design, graphene is served as excellent carrier to improve the catalysis of Mn3(PO4)2 nanoparticles; and DNA adsorbed on graphene acts as medium to assist the growth of Mn3(PO4)2 on graphene. The fabricated graphene/DNA/Mn3(PO4)2 composites exhibit excellently electrochemical activity, significantly decrease the response time and increase the sensitivity of the sensor towards O2-. The successful detection of O2- released from cancer cell demonstrated its potential applications in biology and medicine.