Swine acute diarrhea syndrome coronavirus-related viruses from bats show potential interspecies infection

Abstract

Over the last 20 years, several bat-originated coronaviruses (CoVs), including SARS-CoV, MERS-CoV, and , have caused millions of deaths and severely disrupted global health systems, highlighting the need to investigate bat CoV spillover risks. SADS-CoV, another bat-derived CoV highly pathogenic to piglets, threatens the swine industry and exhibits broad cell tropism, underscoring the need to study these highly diverse viruses with potential for interspecies infection and pathogenicity. As part of our effort to understand these viruses, we developed a framework to characterize them in cell lines and organoids derived from swine and humans, as well as in suckling mice. Additionally, we performed serum cross-neutralization to classify bat SADSr-CoV serotypes, which could guide the development of broad-spectrum vaccines against SADSr-CoVs.