Determination of Various Bioactive Potential of Stoechospermum marginatum (C. Agardh) Kutzing In-Vitro

Abstract

The objective of present study was to screen the antibacterial, total antioxidant and cytotoxic properties of ethanol extract and its fractions of S. marginatum. The highest antibacterial activity was found in ethanol extract followed by dichloromethane, ethyl acetate, hexane and aqueous fractions. TAAs and RAAs were estimated along with known antioxidant such as quercetin, β-carotene and L-ascorbic acid. TAA was found to be highest in EE (22-75%) followed by DMF (18-65%), EAF (13-50%), HF (8-38%) and AF (5-31%). R square value between concentration and TAA of extract/fractions made by linear regression clearly shows TAA dependents on the concentration. Moreover, ethanol extract exhibited cytotoxicity as equal as quercetin at their maximum concentration (640µg/mL and 160µg/mL). Cytotoxicity revealed time-dependent growth inhibition of cancer cells. However, treatment with ethanol extract shows concentration dependent apoptotic cells. The present result and also other recent report clearly exhibited that the S. marginatum having potential antibacterial and antioxidant as well as cytotoxic properties;