Isolation and Structure Elucidation of Bioactive Compounds Chemical as Inhibitors of the Enzyme ?-Glucosidase Raru Bark Ethanol Extract (Vatica pauciflora Blume)

Abstract

Inhibitory compd. of α-glucosidase bioactivity was isolated from ethanol ext. of the stem bark of raru (Vatica pauciflora Blume) which, is a wild plant that grows in Tapanuli state in Indonesia. The ethanol ext. of the stem bark of raru was partitioned and was chromatographed on columns with stationary phase silica gel 60 mesh F254 (0.063 mm-0.200 mm). The ethanol ext. of isolates showed the enzyme α-glucosidase inhibitor IC50 of 93.46. The next step is the detn. of the chem. structure of the UV-visible spectroscopy (UV Vis); FT-IR (Fourier Transform IR spectroscopy), NMR (NMR) namely COSY, Heteronuclear Multiple-Quantum Correlation (HMQC); and Heteronuclear Multiple-Bond Correlation (HMBC). It found of compds. consist of two of methoxy, one of arom., and one of carbonil compds. The isolate is a 3,4,9,Trihidroxy-2-(hydroxymethyl)-8-10-dimethoxy-2-3-4atetrahydropyrano(3,2-c)isochromen-6(10bH)-one according to International Union of Pure and Applied Chem. (IUPAC). [on SciFinder(R)]