Stability of thermostable enzyme, aqualysin I ; a subtilisin-type serine protease from thermus aquaticus YT-1

Abstract

We characterized the heat stability and detergent stabilities of aqualysin I, produced by Thermus aquaticus YT-1, and compared them with those of fungal proteinase K and Bacillus subtilisin.Aqualysin I displayed excellent heat and detergent stabilities. Proteinase K, another Cys-containing enzyme, was less stable than aqualysin I. All these enzymes maintained activities in the presence of urea or Tween-20. © 1998, Taylor & Francis Group, LLC. All rights reserved.