Spatial organization and dynamics of the extracellular space in the mouse retina

Abstract

The extracellular space (ECS) plays an important role in the physiology of neural circuits. Despite our detailed understanding of the cellular architecture of the mammalian retina, little is known about the organization and dynamics of the retinal ECS. We developed an optical technique based on two-photon imaging of fluorescently labeled extracellular fluid to measure the ECS volume fraction (a) in the ex vivo retina of male and female mice. This method has high spatial resolution and can detect rapid changes in a evoked by osmotic challenge and neuronal activity. The measured ECS a varied dramatically in different layers of the adult mouse retina, with a equaling;0.050 in the ganglion cell layer,;0.122 in the inner plexiform layer (IPL),;0.025 in the inner nuclear layer (INL),;0.087 in the outer plexiform layer, and;0.026 in the outer nuclear layer (ONL). ECS a was significantly larger early in retinal development; a was 67% larger in the IPL and 100% larger in the INL in neonatal mice compared with adults. In adult retinas, light stimulation evoked rapid decreases in ECS a. Light-driven reductions in ECS a were largest in the IPL, where visual stimuli decreased a values;10%. These light-evoked decreases demonstrate that a physiological stimulus can lead to rapid changes in ECS a and indicate that activity-dependent regulation of extracellular space may contribute to visual processing in the retina.