Adaptation of acid-tolerant Leucaena leucocephala cv Tarramba Calluses after gamma irradiation on regenerative media

Abstract

The aim of this study to measure adaptation of callus Lamtoro (Leucaena leucocephala cv. Tarramba) after gamma irradiation on regenerative media. Lamtoro plant breeding in the form of callus with gamma ray irradiation is required for the development of a new plant-tolerant strain line with a gene mutation approach in tissue culture. The experimental design in this study was a complete randomized design (CRD). There were 13 treatments consitsing of control (M0). 6-benzyl amino purine (BAP) media (M1-M4). kinetin media (M5-M8). and combination of BAP and kinetin media (M9-M12) during 8 weeks. The measured variables were viability of callus. the addition of diameter and height callus. colour and texture callus. Data were tested using analysis of variance (ANOVA) and continued with Duncan test if there were a significant difference among treatment. Data of viability colour and texture used descriptive analysis. The data showed that formulation media M12 (combination 1 mg L -1 BAP and 1 mg L -1 kinetin) was significantly different (P < 0.05) in 8 week after plant (WAP). It was concluded that the best regenerative media for adaptation of callus lamtoro was combination of BAP and kinetin media.