Phytochemical and pharmacological profiling of Trewia nudiflora Linn. leaf extract deciphers therapeutic potentials against thrombosis, arthritis, helminths, and insects

Abstract

The objective of the current study was to examine the phytochemical and in vitro thrombolytic, anti-arthritic, anthelmintic, and insecticidal effects of Trewia nudiflora (TN) methanolic leaf extract with its methanol (MTN), chloroform (CTN), and ethyl acetate (ETN) fractions. Pheretima posthuma and Tribolium castaneum were employed for evaluating the antihelmintic and insecticidal properties, respectively. All the tested extracts showed the presence of copious potential constituents in phytochemical analysis. Among all extracts, MTN extract exhibited the utmost clot lysis (35.95 ± 4.81%) property compared to standard streptokinase (SK) (53.77 ± 7.52%). All samples displayed striking protein denaturation activity in a dose-dependent manner (100-500 μg/mL), where the highest inhibition was observed for MTN (67.26 ± 6.39% at 500 μg/mL). Each extract demonstrated considerable anthelmintic activity at 25-75 mg/mL dose ranges. ETN showed the strongest anthelmintic activity at the highest dose. Among all samples, the CTN extract displayed the utmost mortality rate (77.22%) in the insecticidal test. The results of the study suggest that T. nudiflora leaf extracts may have potential against thrombosis, arthritis, helminths, and insects, which warrants the necessity of extensive isolation and identification of bioactive compounds to develop newer effective drugs upon preclinical and clinical investigations.