Structural Investigation of Laminaran of Eisenia bicyclis

Abstract

Laminaran was isolated from Eisenia bicyclis. On the basis of the methylation, periodate oxidation, NMR spectroscopy by 1Hand 13 Cnuclei, this glucan was shown to have a branched structure composed of (l→3)— and (l→6)-Iinked β-D-glucopyranose residues in the ratio 3: 2. The glucan was especially characterized by comparison of its 13C and 1H NMR spectra with those of related compounds. The NMR investigation of the polyol polysaccharide, ob-tained by reduction after periodate oxidation of laminaran, was very useful for elucidation of the composition, structure and major sequence of the native glucan. © 1979, Japan Society for Bioscience, Biotechnology, and Agrochemistry. All rights reserved.