Comparison of five RNA extraction methods from rabbit’s blood

Abstract

Extraction of RNA from eukaryotic cells is a crucial stage in many molecular biology studies. Thusit is essential to use the most appropriate method that maximizes the yield and maintains theintegrity of extracted RNA. The present work compared five manual RNA extraction methods ofdifferent dilutions of rabbit blood. The assessment of the concentration and purity of the extractedRNA were used as parameters to evaluate the efficiency of each method. Statistical analysisshowed that the most effective method for RNA extraction from rabbit blood is by following theprotocol of the RNeasy Lipid Tissue kit, but it requires the use of toxic reagents. The mostefficient kit using no toxic reagents is the RNeasy protect animal blood.