Isolation and Identification of Bioactive Proteins from Microsymbiont Algae Eucheuma cottonii and Their Potential as An Anticancer

Abstract

The isolation and identification of bioactive proteins from the microsymbiont algae Eucheuma cottoni from Laikang district of Takalar in South Sulawesi have been carried out. This study aimed to isolate and identify the bioactive proteins from microsymbiont algae E. cottonii and their potential as an anticancer. The experiment method includes the stages of extraction, fractionation, and dialysis. The obtained bioactive proteins were identified by determinating protein contents using the Lowry method. Furthermore, the preliminary anticancer test was conducted by testing Brine Shrimp Lethality Test (BSLT) method against shrimp larvae Artemia salina Leach. and the antimitotic test was also conducted against the sea urchin Tripneustes gratilla Linn. zygote cells. The result showed that the protein fraction of 40-60% saturation was the most toxic with LC50 with a value of 91.83 μg/mL and IC50 with a value of 74.13 μg/mL. This result suggested that the bioactive proteins of microsymbiont algae E. cottonii could be used as a potential anticancer agent.