(+)-EPOXYDONE: A MAJOR SECONDARY METABOLITE AS ANTIBACTERIAL AGENT FROM Phomopsis sp. TcBt1Bo-6 ISOLATED FROM STEM OF BROTOWALI PLANT (Tinospora crispa)

Abstract

Isolation, characterization, and bioproduction capacity of (+)-epoxydone produced by Phomopsis sp. TcBt1Bo-6 was performed in this present report. The fungus was cultured in potatoes dextrose broth (PDB) medium. Isolation and purification of (+)-epoxydone using chromatography methods. The metabolite structure elucidation was carried out by using UV, FTIR, 1D/2D-NMR, MS, and optical rotation, and compared with other reports. This fungus has the bioproduction ability of (+)-epoxydone of 189.43 mg/L. The metabolite of (+)-epoxydone has potent as antibacterial and antimycobacterial against E.coli, S.aureus, and M.smegmatis with MIC values of 25, > 50, and 50 (partial) μg/mL, respectively. This study is the new report on the bioproduction of bioactive metabolite (+)-epoxydone as well as the antibacterial and antimycobacterial activities of this compound.