Phytochemical and antifungal activity combination of Costus speciosus rhizome and Bryophyllum pinnatum in vitro

Abstract

The herbs use plants from Dayak ethnicity often used to solve infertility for women. The plants are Costus specious and Bryophllum pinnatum. This study aimed to determine the content of compounds present in the combination of Costus specious and Bryophllum pinnatum and the antifungal activity of Candida albicans. This research is qualitative descriptive. Costus specious and Bryophllum pinnatum extracted using a single maceration method with a water solvent. Phytochemical tests used the Meyer Test method, Dragendorf Test for alkaloids, Wilstater Test for flavonoids, Lieberman Burchard Test for triterpenoids, Forth Test for saponins, and FeCl3 Test for tannins. Antifungal activity test of Candida albicans used in vitro test the 100% diffusion method and microdilution with a concentration of 50%, 25%, 12.5%, 6.25%, 3.13%, 1.56%, 0.78%, and 0.39%. The results of phytochemical tests showed that the combination of aqueous extracts of Costus specious and Bryophllum pinnatum is flavonoids and triterpenoids. The antifungal test showed that the combination extract inhibited the growth of Candida albicans with an inhibitory zone of 8.91 (moderate), 10.42 (moderate), and 8.23 (moderate). The MIC value at a concentration of 1.56% and the KBM value at a concentration of 3.13%.