Post-thaw thermal resistance test on motility and acrosomal integrity of filtered and non-filtered frozen semen of Murrah buffalo bulls

Abstract

Present investigation was conducted to determine the post-thaw sperm motility and acrosomal damage of filtered and non-filtered frozen semen of Murrah buffalo bulls. Twenty semen ejaculates (from four Murrah buffalo bulls collected at weekly interval) were diluted in Tris egg yolk glycerol extender and divided into two parts. One was filtered through sephadex G-100 column and the other portion was kept as such (non-filtered). Both fractions were frozen in liquid nitrogen (-196°C) by the standard method developed in the laboratory. After 24 h of freezing, non-filtered and filtered semen samples were thawed at 37°C for 1 min. These samples were incubated at 37°C in a water both. The different seminal characteristics i.e. percent progressive sperm motility, live and abnormal spermatozoa and spermatozoa with damaged acrosome were assessed at hourly interval till they remained motile. The filtered frozen and thawed semen showed significantly (p<0.05) high sperm viability and acrosomal integrity as compared to non-filtered semen.