Vaccine-induced immunity against cutaneous Leishmaniasis in BALB/c mice

Abstract

Partially purified antigens, derived from Leishmania infantum or L. major promastigot and isolated under reducing conditions, were used to immunize BALB/c mice. Three subcutaneous injections of the 64- to 97-kilodalton preparation in conjunction with muramyl dipeptide conferred long-lasting immunity against L. mexicana subsp. mexicana and L. major infection; they led to the development of antibodies neutralizing the infectiousness of promastigotes, induced specific delayed-hypersensitivity reactions, and generated populations of peritoneal macrophages capable of killing amastigotes. Vaccination resulted in no harmful effects, since these antigens neither exacerbated preexisting Leishmania infection nor impeded the formation of antibodies to other antigens administered concomitantly.