Fluctuation in O-GlcNAcylation inactivates STIM1 to reduce store-operated calcium ion entry via down-regulation of Ser621phosphorylation

Abstract

Stromal interaction molecule 1 (STIM1) plays a pivotal role in store-operated Ca2+entry (SOCE), an essential mechanism in cellular calciumsignaling and in maintaining cellular calcium balance. Because O-GlcNAcylation plays pivotal roles in various cellular function, we examined the effect of fluctuation in STIM1 O-GlcNAcylation on SOCE activity. We found that both increase and decrease in STIM1 O-GlcNAcylation impaired SOCE activity. To determine the molecular basis, we established STIM1-knockout HEK293 (STIM1-KO-HEK) cells using the CRISPR/Cas9 system and transfected STIM1 WT (STIM1-KOWT- HEK), S621A (STIM1-KO-S621A-HEK), or T626A (STIM1- KO-T626A-HEK) cells. Using these cells, we examined the possible O-GlcNAcylation sites of STIM1 to determine whether the sites were O-GlcNAcylated. Co-immunoprecipitation analysis revealed that Ser621and Thr626were O-GlcNAcylated and that Thr626was O-GlcNAcylated in the steady state but Ser621was not. The SOCE activity in STIM1-KO-S621A-HEK and STIM1- KO-T626A-HEK cells was lower than that in STIM1-KO-WTHEK cells because of reduced phosphorylation at Ser621. Treatment with the O-GlcNAcase inhibitor Thiamet G or O-GlcNAc transferase (OGT) transfection, which increases O-GlcNAcylation, reduced SOCE activity, whereas treatment with the OGT inhibitor ST045849 or siOGT transfection, which decreases OGlcNAcylation, also reduced SOCE activity. Decrease in SOCE activity due to increase and decrease in O-GlcNAcylation was attributable to reduced phosphorylation at Ser621. These data suggest that both decrease in O-GlcNAcylation at Thr626and increase in O-GlcNAcylation at Ser621in STIM1 lead to impairment of SOCE activity through decrease in Ser621phosphorylation. Targeting STIM1 O-GlcNAcylation could provide a promising treatment option fo.