Hardware for cell culture electrical impedance tomography: A critical review

Abstract

Human cell cultures are powerful laboratory tools for biological models of diseases, drug development, and tissue engineering. However, the success of biological experiments often depends on real-time monitoring of the culture state. Conventional culture evaluation methods consist of end-point laborious techniques, not capable of real-time operation and not suitable for three-dimensional cultures. Electrical Impedance Tomography (EIT) is a non-invasive imaging technique with high potential to be used in cell culture monitoring due to its biocompatibility, non-invasiveness, high temporal resolution, compact hardware, automatic operation, and high throughput. This review approaches the different hardware strategies for cell culture EIT that are presented in the literature, discussing the main components of the measurement system: excitation circuit, voltage/current sensing, switching stage, signal specifications, electrode configurations, measurement protocols, and calibration strategies. The different approaches are qualitatively discussed and compared, and design guidelines are proposed.